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The papers in this volume were part of the technical conference cited on the cover and title page. Papers were selected and subject to review by the editors and conference program committee. Some conference presentations may not be available for publication. Additional papers and presentation recordings may be available online in the SPIE Digital Library at SPIEDigitalLibrary.org. The papers reflect the work and thoughts of the authors and are published herein as submitted. The publisher is not responsible for the validity of the information or for any outcomes resulting from reliance thereon. Please use the following format to cite material from these proceedings: Author(s), “Title of Paper,” in Multiphoton Microscopy in the Biomedical Sciences XVII, edited by Ammasi Periasamy, Peter T. So, Karsten König, Xiaoliang S. Xie, Proceedings of SPIE Vol. 10069 (SPIE, Bellingham, WA, 2017) Seven-digit Article CID Number. ISSN: 1605-7422 ISSN: 2410-9045 (electronic) ISBN: 9781510605794 ISBN: 9781510605800 (electronic) Published by SPIE P.O. Box 10, Bellingham, Washington 98227-0010 USA Telephone +1 360 676 3290 (Pacific Time) · Fax +1 360 647 1445 Copyright © 2017, Society of Photo-Optical Instrumentation Engineers. Copying of material in this book for internal or personal use, or for the internal or personal use of specific clients, beyond the fair use provisions granted by the U.S. Copyright Law is authorized by SPIE subject to payment of copying fees. The Transactional Reporting Service base fee for this volume is $18.00 per article (or portion thereof), which should be paid directly to the Copyright Clearance Center (CCC), 222 Rosewood Drive, Danvers, MA 01923. Payment may also be made electronically through CCC Online at copyright.com. Other copying for republication, resale, advertising or promotion, or any form of systematic or multiple reproduction of any material in this book is prohibited except with permission in writing from the publisher. The CCC fee code is 1605-7422/17/$18.00. Printed in the United States of America. Publication of record for individual papers is online in the SPIE Digital Library.
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AuthorsNumbers in the index correspond to the last two digits of the seven-digit citation identifier (CID) article numbering system used in Proceedings of SPIE. The first five digits reflect the volume number. Base 36 numbering is employed for the last two digits and indicates the order of articles within the volume. Numbers start with 00, 01, 02, 03, 04, 05, 06, 07, 08, 09, 0A, 0B…0Z, followed by 10-1Z, 20-2Z, etc. Abi Haidar, Darine, 2M Abi Lahoud, Georges, 2M Appleton, Paul, 2F Atsuta, K., 2B Ballmann, Charles W., 0Z Barbano, Emerson C., 2P Barbieri, Beniamino, 18, 1C, 1X Barroso, Margarida, 17 Bartels, Randy A., 1P, 2P Batista, Ana, 06, 1Y Becker, Wolfgang, 16 Beier, Hope T., 1B Berent, Zachary T., 08 Biggs, David S. C., 08 Boudoux, Caroline, 27 Bouhabel, Sarah, 2E Bouman, Charles A., 12 Breunig, Hans Georg, 1Y Brunton, V. G., 0T Bruschini, Claudio, 1S Callis, Patrik R., 20 Chandler, Aaron, 2R Chandler, Andrea, 2R Chandra, Dhyan, 1L Charan, Kriti, 0Y Charbon, Edoardo, 1S Chen, Andy J., 11 Chen, Jianxin, 1R, 2J Chen, Xiuqiang, 2J Chen, Zhida, 2J Cheng, Ji-Xin, 11, 12 Chicco, Adam J., 1P Christopher, Kathryn G., 1L Cicchi, R., 0B Coskun, Ulas C., 18, 1C, 1X Cruz-Hernandez, Jean C., 2L Cui, Sishan, 0M Danielius, Romualdas, 2T Dantus, Marcos, 2K de Reguardati, Sophie, 20 Devaux, Bertrand, 2M Diaspro, Alberto, 1C Domingue, Scott R., 1P Dong, Chen-Yuan, 1J Douillette, Annie H., 2E Dudenkova, Varvara V., 1K, 2G Dunn, Andrew K., 0C Eakins, Gregory, 11 Eibl, Matthias, 1F Feng, Danielle, 2L Ferreon, Allan Chris, 1X Ferreon, Josephine C., 1X Field, Jeffrey J., 2P Finnøy, Andreas, 2I Fried, Glenn A., 08 Fu, Yu Vincent, 2J Furman, Olga E., 1K Gartia, Manas Ranjan, 08 Gavrina, Alena I., 1K George, Steven C., 18 Godbout, Nicolas, 27 Gorzelanny, Christian, 1E Hagan, Kristen, 0C Hager, Tobias, 06 Hakert, Hubertus, 1F Hakulinen, T., 0D Hanninen, Adam M., 2H Hase, E., 09, 2B Hassan, Ahmed, 0C He, Sicong, 0O Homulle, Harald, 1S Hong, Weili, 11 Hoy, Christopher, 2P Hu, Rui, 22 Huang, Jiandong, 0O Huang, Kai-Chih, 11, 12 Huber, Robert, 1F Huck, Volker, 1E Hulme, A. N., 0T Ibey, Bennett L., 1B Ibrahim, Ali, 2M Intes, Xavier, 17 Ishitsuka, Yuji, 1C Kabir, Mohammad M., 08 Kalinina, S., 1I Kang, Deyong, 1R Kang, Ning, 1J Karanja, Caroline, 11 Karpf, Sebastian, 1F Kazarine, Alexei, 2E Kazmi, S. M. Shams, 0C Klein, J., 0D Klementieva, Natalia V., 1K Kohler, Bern, 20 Kong, Nan, 12 König, Karsten, 06, 1E, 1Y Korfanty, E. R., 15 Kost, Karen, 2E Lam, Sandra, 18 Leduc, Mikaël, 27 Lee, Hsuan-Shu, 1J Lee, Jian-Ye, 1J Lee, M., 0T Li, Bo, 0Y Li, Jingting, 14 Li, Xuesong, 0O Liao, Chien-Sheng, 11, 12 Liao, Shih-Chu Jeff, 18, 1C, 1X Li-Jessen, Nicole Y. K., 2E Lilledahl, Magnus B., 2I Lin, Chih-Ju, 1J Lin, Danying, 22 Lin, Haonan, 12 Lin, Hung-Ming, 1J Lin, Jiangbo, 1R Lin, Peihua, 2J Lin, Ruolan, 2J Lindquist, Jake R., 20 Liu, Gang Logan, 08 Luger, Thomas A., 1E Lukina, Maria M., 1K, 2G Lukyanov, Konstantin A., 1K Majeau, Lucas, 27 Malandrini, A., 0B Mancuso, Christopher A., 2K Mazurkiewicz, Joseph E., 17 Medina, Flor A., 0C Melouki, Fatima, 2M Menabuoni, L., 0B Meng, Zhaokai, 0Z Mercatelli, R., 0B Mess, Christian, 1E Metze, Dieter, 1E Mikhailov, Alexander, 20, 24 Miller, David R., 0C Minamikawa, T., 09, 2B Mirkhanov, Shamil, 2F Mishin, Alexander S., 1K Moger, Julian, 28 Mongeau, Luc, 2E Murashova, Gabrielle A., 2K Nicoletti, R., 0B Nishimura, Nozomi, 2L Ogura, Y., 2B Olstad, Kristin, 2I Orlova, Natalia, 1T Ouzounov, Dimitre G., 2L Pahapill, Juri, 20 Palczewska, Grazyna, 2K Palczewski, Krzysztof, 2K Pallud, Johan, 2M Pavone, F. S., 0B Penfield, Steven, 28 Peng, Xiao, 22 Perelman, Gregory S., 1K Periasamy, Ammasi, 1L, 2R Perillo, Evan P., 0C Perrillat-Bottonet, Thomas, 27 Pini, R., 0B Porquez, J. G., 15 Potma, Eric O., 2H Poulon, Fanny, 2M Praveen, Bavishna B., 2F Qi, Jing, 22 Qiu, Ping, 1Q, 29 Qu, Jianan Y., 0O Qu, Junle, 22 Quarterman, Adrian H., 2F Rammo, Matt, 20 Ratto, F., 0B Rebane, Aleksander, 20, 24 Rehman Alam, Shagufta, 1L Reimer, Jacob, 2L Rossi, F., 0B Rück, Angelika C., 1I Rudkouskaya, Alena, 17 Sadayappan, Sakthivel, 08 Saggau, Peter, 1T Sakami, Sanae, 2K Sato, K., 09 Schäfer, P., 1I Schneider, Stefan W., 1E Sedelnikova, Anna, 1B Seitz, Berthold, 06 Selvin, Paul R., 1C Sergeeva, Tatiana F., 1K Serrels, A., 0T Shih, Wei-Chuan, 14 Shirmanova, Marina V., 1K, 2G Shumilova, Anastasia, 2G Sinefeld, David, 0Y Sinsuebphon, Nattawut, 17 Sivaguru, Barghav S., 08 Sivaguru, Mayandi, 08 Sivaguru, Vignesh A., 08 Slepkov, A. D., 15 Smietana, Stefan, 16 Smyth, Connor J. C. P., 2F Snopova, Ludmila B., 2G Squier, Jeff, 2P Stasevicius, Ignas, 2T Stoltzfus, Caleb, 24 Strupler, Mathias, 27 Sun, Qiqi, 0O Sun, Yuansheng, 18, 1C, 1X Svindrych, Zdenek, 1L Swift, Samuel, 2F Takahashi, M., 09 Tatini, F., 0B Teng, Kai-Wen, 1C Thomson, Calum, 2F Tipping, W. J., 0T Tolias, Andreas, 2L Tolstykh, Gleb P., 1B Tortarolo, Giorgio, 1C Toussaint, Kimani C., Jr., 08 Trummal, Aleksander, 20 Tsoi, Phoebe S., 1X Tsyboulski, Dmitri, 1T Uchugonova, Aisada, 1Y Uudsemaa, Merle, 20 Varlet, Pascale, 2M Vengris, Mikas, 2T Vicidomini, Giuseppe, 1C von Arnim, C., 1I von Einem, B., 1I Wagoner Johnson, Amy J., 08 Wallrabe, Horst, 1L, 2R Walsh, Alex J., 1B Wang, Chun-Chin, 28 Wang, Ke, 0Y Wang, Mengran, 2L Wang, Ping, 0M Wang, Pu, 11, 12 Wang, Shu, 2J Wang, Tianyu, 2L Wang, Xingfu, 2J Wen, Wenhui, 1Q, 29 Weng, Daniel, 1F Wernsing, Keith A., 2P Wilcox, Keith G., 2F Wilson, Jesse W., 1P Wiseman, Paul W., 2E Worts, Nathan, 2P Wu, Qianqian, 22 Wu, Weilin, 2J Xu, Chris, 0Y, 2L Xu, Jian, 1R Xue, Wenqian, 0O Yakovlev, Vladislav V., 0Z Yan, Wei, 22 Yasui, T., 09, 2B Yonekura, D., 09 Yoong, Fei-Yian, 28 Young, Michael D., 2P Yue, Shuhua, 0M Yue, Yuankai, 14 Zagaynova, Elena V., 1K, 2G Zanello, Marc, 2M Zemelman, Boris V., 0C Zens, Katharina, 1E Zhu, Xiaoqin, 1R Zhuo, Shuangmu, 1R Zlobovska, Olga A., 1K Conference CommitteeSymposium Chairs
Program Track Chairs
Conference Chairs
Conference Program Committee
Session Chairs
IntroductionMultiphoton microscopy has been established as the 3D imaging method of choice for studying biomedical specimens from single cells and whole animals to patients with sub-micron resolution. 26 years have passed since the realization of two-photon laser scanning microscopy. The ever-expanding scope of applications and the continuing instrumental innovations require a forum where new ideas can be exchanged and presented. Our conference at the SPIE Photonics West meeting continues to address this need. This is the 17th year of this conference, and it began with four keynote lectures from leaders in the field:
For the sixth year in a row, the conference included the JenLab Young Investigator Award, in addition to our regular poster awards. The JenLab Young Investigator Award is sponsored by JenLab GmbH (Germany). The award selection committee included Drs. Arnd Krueger, Newport-Spectra Physics, (United States), Francesca Pavone, University of Florence (Italy), Paul Campagnola, University of Wisconsin-Madison, (United States), Conor Evans, Massachusetts General Hospital, (United States), and the conference chairs. The selection process included reviewing seven abstracts, manuscripts, and poster presentations. Two finalists were selected for oral presentation after their poster presentation. The two finalists were:
The review panel selected Mr. Tianyu Wang, Cornell University, (United States) as the winner of the JenLab Young Investigator Award 2017. The winner received a certificate and $2000. This year, for the first time, we gave a certificate and $500 award for the runner-up. The awards were presented by Dr. Karsten König, President and Founder of JenLab GmbH, Germany. For 17 years in a row, the conference organized poster awards for the students and postdoctoral fellows. The poster award was donated by all of the conference sponsors including: Applied Scientific Instruments (ASI), Becker & Hickl, Carl Zeiss, Chroma Technology, Coherent, ISS Inc., Leica Microsystems, Semrock (IDEX), and Spectra Physics (A Newport Company). The 4 poster award winners were:
Some of the most valuable contributions in this volume are articles written by highly experienced practitioners of multiphoton microscopy. They have enumerated the most important considerations in designing multiphoton microscopes and imaging experiments. Further, updates on the state-of-the-art commercial multiphoton microscope systems are included. This volume also features articles describing some recent advances in major multi-photon microscope components and applications including laser light sources, ultrafast optics, filters, FRET, FLIM, FCS, Raman, CARS, SRS and CRS microscopy and spectroscopy, single molecule, endoscopy, in vivo/intravital imaging, metabolism measurements including NADH, FAD, tryptophan in cells and tissues, and various scientific and clinical applications. On a personal note, the conference chairs are grateful for the participation of all authors and session chairs and acknowledge the innovation-driven manufacturers and sponsors of this conference for their enthusiastic support in organizing this conference successfully for the last 16 years. We look forward to other exciting conferences in the future and welcome your continued participation and support. Ammasi Periasamy Peter T. C. So Karsten König Xiaoliang S. Xie |
