21 February 2017 In vivo multiphoton microscopy beyond 1 mm in the brain
Author Affiliations +
We perform high-resolution, non-invasive, in vivo deep-tissue imaging of the mouse neocortex using multiphoton microscopy with a high repetition rate optical parametric amplifier laser source tunable between λ=1,100 and 1,400 nm. We demonstrate an imaging depth of 1,200 μm in vasculature and 1,160 μm in neurons. We also demonstrate deep-tissue imaging using Indocyanine Green (ICG), which is FDA approved and a promising route to translate multiphoton microscopy to human applications.
Conference Presentation
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
David R. Miller, David R. Miller, Flor A. Medina, Flor A. Medina, Ahmed Hassan, Ahmed Hassan, Evan P. Perillo, Evan P. Perillo, Kristen Hagan, Kristen Hagan, S. M. Shams Kazmi, S. M. Shams Kazmi, Boris V. Zemelman, Boris V. Zemelman, Andrew K. Dunn, Andrew K. Dunn, } "In vivo multiphoton microscopy beyond 1 mm in the brain", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690C (21 February 2017); doi: 10.1117/12.2249949; https://doi.org/10.1117/12.2249949

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