21 February 2017 In vivo multiphoton imaging and quantification of cytoplasmic and nuclear metabolism in the hepatobiliary system
Author Affiliations +
Abstract
Liver performs xenobiotic excretion out of hepatocytes with metabolic function. However, hepatocellular metabolism was non-uniform in hepatocyte. Hepatocellular metabolism could be different in nucleus and cytoplasm. In this study, we use the molecular probe 6-carboxyfluorescein diacetate (6-CFDA) to simulate xenobiotic metabolism in hepatocytes with multi-photon fluorescence microscopy in vivo. 6-CFDA was processed by intracellular esterase to 6- carboxyfluorescein (6-CF) with green fluorescence. And this probe was used to study differences in cytoplasmic and nuclear metabolism of hepatocytes.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Chih-Ju Lin, Chih-Ju Lin, Ning Kang, Ning Kang, Jian-Ye Lee, Jian-Ye Lee, Hung-Ming Lin, Hung-Ming Lin, Hsuan-Shu Lee, Hsuan-Shu Lee, Chen-Yuan Dong, Chen-Yuan Dong, } "In vivo multiphoton imaging and quantification of cytoplasmic and nuclear metabolism in the hepatobiliary system", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691J (21 February 2017); doi: 10.1117/12.2254944; https://doi.org/10.1117/12.2254944
PROCEEDINGS
6 PAGES


SHARE
Back to Top