21 February 2017 Identification of intramural metastasis in esophageal cancer using multiphoton microscopy
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Intramural metastasis (IM) of esophageal cancer is defined as metastasis from a primary lesion to the esophageal wall without intraepithelial cancer extension. Esophageal cancer with IM is more common and such cases indicate a poor prognosis. In esophageal surgery, if curative resection is possible, the complete removal of both primary tumor and associated IMs is required. Therefore, accurate diagnosis of IMs in esophageal cancer prior to surgery is of particular importance. Multiphoton microscopy (MPM) with subcellular resolution is well-suited for deep tissue imaging since many endogenous fluorophores of fresh biological tissues are excited through two-photon excited fluorescence (TPEF) and second harmonic generation (SHG). Here, a study to identify IM in fresh tissue section using MPM is reported. In this study, the morphological and spectral differences between IM and surrounding tissue are described. These results show that MPM has the ability to accurately identify IM in esophageal tissues. With improvement of the penetration depth of MPM and the development of multiphton microendoscope, MPM may be a promising imaging technique for preoperative diagnosis of IMs in esophageal cancer in the future.
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Jian Xu, Jian Xu, Deyong Kang, Deyong Kang, Shuangmu Zhuo, Shuangmu Zhuo, Xiaoqin Zhu, Xiaoqin Zhu, jiangbo Lin, jiangbo Lin, Jianxin Chen, Jianxin Chen, } "Identification of intramural metastasis in esophageal cancer using multiphoton microscopy", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691R (21 February 2017); doi: 10.1117/12.2250766; https://doi.org/10.1117/12.2250766

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