2 March 2017 Rapid hyperspectral, vibrationally resonant sum-frequency generation microscopy
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We discuss the development and application of a laser-scanning, nonlinear optical microscope capable of generating vibrationally resonant images based on sum-frequency generation (SFG), coherent anti-Stokes Raman scattering (CARS) or third-order sum-frequency generation (TSFG). The combination of these three modalities allows vibrationally sensitive imaging of both χ (2) and χ (3)-active structures in biological tissues, addressing both Raman-active as well as IR-allowed vibrational modes. We show the practical utility of these vibrationally sensitive modalities by imaging collagen I rich tissues.
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Adam M. Hanninen, Adam M. Hanninen, Eric O. Potma, Eric O. Potma, } "Rapid hyperspectral, vibrationally resonant sum-frequency generation microscopy", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692H (2 March 2017); doi: 10.1117/12.2253295; https://doi.org/10.1117/12.2253295

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