17 February 2017 Toward multi-focal spot remote focusing two-photon microscopy for high speed imaging
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Abstract
Optical sectioning techniques using two-photon excitation of fluorescent indicators are central to diverse imaging applications. The limitations of the technique are low speed and undesirable specimen agitation. In our design, high-speed axial scanning is carried out by moving a reference objective to axially displace the focal spot without introducing significant spherical aberration and any agitation of the specimen. Further, the system is configured to allow switching between single spot and multiple focal spot remote focusing to allow either high dynamic range or high speed imaging.
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Bei Li, Bei Li, Alexander D. Corbett, Alexander D. Corbett, Ee Chong, Ee Chong, Edward Mann, Edward Mann, Tony Wilson, Tony Wilson, Martin J. Booth, Martin J. Booth, Gil Bub, Gil Bub, } "Toward multi-focal spot remote focusing two-photon microscopy for high speed imaging", Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 1007005 (17 February 2017); doi: 10.1117/12.2251214; https://doi.org/10.1117/12.2251214
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