22 March 2017 Single image sectioning using spatial frequency domain imaging
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Abstract
Structured illumination microscopy (SIM) works well to produce depth information by removing undesired light from out of focus planes within a specimen. However, it generally requires multiple images in order to produce sectioning. We show that, under the right conditions, we can leverage Spatial Frequency Domain Imaging (SFDI) to produce both sectioning and relative depth information about a specimen using only a single image. After processing, we find that the axial resolution is comparable to that of traditional 3 phase SIM. With this technique, we are able to produce real-time videos in-vivo at 21 frames per second. Additionally, no moving parts are needed to produce sectioning at depth.
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Zachary R. Hoffman, Zachary R. Hoffman, Charles A. DiMarzio, Charles A. DiMarzio, } "Single image sectioning using spatial frequency domain imaging", Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 1007014 (22 March 2017); doi: 10.1117/12.2254734; https://doi.org/10.1117/12.2254734
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