24 February 2017 Laser speckle contrast imaging augmented with structured light
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Abstract
This paper presents a new method of blood flow imaging in vessels within and underneath the skin. The combination of Laser Speckle Contrast Imaging (LSCI) and Spatial Frequency Domain Imaging (SFDI)/ Structured Illumination Microscopy (SIM) allows one to retrieve a substantial amount of flow information, which is otherwise occluded by the skin. In this article, the experiment consists of illuminating a phantom with coherent light passing through a grid pattern which is modulated at the plane of interest. The speckle pattern is then imaged and processed using a combination of structured illumination sectioning and laser speckle contrast. The whole process is realized using an inexpensive setup consisting of a laser diode (Wavelength = 758 nm), mirrors, lenses, a grid and an 8-bit camera. This advanced, yet easy to implement, technique will allow deep skin imaging to be fast, effective and inexpensive.
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Jaime Necochea, Jaime Necochea, Jaime Prieto, Jaime Prieto, Zachary Hoffman, Zachary Hoffman, Charles DiMarzio, Charles DiMarzio, } "Laser speckle contrast imaging augmented with structured light", Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 100701F (24 February 2017); doi: 10.1117/12.2275272; https://doi.org/10.1117/12.2275272
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