21 February 2017 Molecular counting of membrane receptor subunits with single-molecule localization microscopy
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We report on quantitative single-molecule localization microscopy, a method that next to super-resolved images of cellular structures provides information on protein copy numbers in protein clusters. This approach is based on the analysis of blinking cycles of single fluorophores, and on a model-free description of the distribution of the number of blinking events. We describe the experimental and analytical procedures, present cellular data of plasma membrane proteins and discuss the applicability of this method.
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Carmen Krüger, Carmen Krüger, Franziska Fricke, Franziska Fricke, Christos Karathanasis, Christos Karathanasis, Marina S. Dietz, Marina S. Dietz, Sebastian Malkusch, Sebastian Malkusch, Gerhard Hummer, Gerhard Hummer, Mike Heilemann, Mike Heilemann, } "Molecular counting of membrane receptor subunits with single-molecule localization microscopy", Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 100710K (21 February 2017); doi: 10.1117/12.2256638; https://doi.org/10.1117/12.2256638

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