2 March 2017 16-Ch time-resolved single-molecule spectroscopy using line excitation
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Abstract
Single-molecule spectroscopy on freely-diffusing molecules allows detecting conformational changes of biomolecules without perturbation from surface immobilization. Resolving fluorescence lifetimes increases the sensitivity in detecting conformational changes and overcomes artifacts common in intensity-based measurements. Common to all freely-diffusing techniques, however, are the long acquisition times. We report a time-resolved multispot system employing a 16-channel SPAD array and TCSPC electronics, which overcomes the throughput issue. Excitation is obtained by shaping a 532 nm pulsed laser into a line, matching the linear SPAD array geometry. We show that the line-excitation is a robust and cost-effective approach to implement multispot systems based on linear detector arrays.
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Antonino Ingargiola, Pietro Peronio, Eitan Lerner, Angelo Gulinatti, Ivan Rech, Massimo Ghioni, Shimon Weiss, Xavier Michalet, "16-Ch time-resolved single-molecule spectroscopy using line excitation", Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 100710Q (2 March 2017); doi: 10.1117/12.2256367; https://doi.org/10.1117/12.2256367
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