2 March 2017 16-Ch time-resolved single-molecule spectroscopy using line excitation
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Abstract
Single-molecule spectroscopy on freely-diffusing molecules allows detecting conformational changes of biomolecules without perturbation from surface immobilization. Resolving fluorescence lifetimes increases the sensitivity in detecting conformational changes and overcomes artifacts common in intensity-based measurements. Common to all freely-diffusing techniques, however, are the long acquisition times. We report a time-resolved multispot system employing a 16-channel SPAD array and TCSPC electronics, which overcomes the throughput issue. Excitation is obtained by shaping a 532 nm pulsed laser into a line, matching the linear SPAD array geometry. We show that the line-excitation is a robust and cost-effective approach to implement multispot systems based on linear detector arrays.
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Antonino Ingargiola, Antonino Ingargiola, Pietro Peronio, Pietro Peronio, Eitan Lerner, Eitan Lerner, Angelo Gulinatti, Angelo Gulinatti, Ivan Rech, Ivan Rech, Massimo Ghioni, Massimo Ghioni, Shimon Weiss, Shimon Weiss, Xavier Michalet, Xavier Michalet, } "16-Ch time-resolved single-molecule spectroscopy using line excitation", Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 100710Q (2 March 2017); doi: 10.1117/12.2256367; https://doi.org/10.1117/12.2256367
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