21 February 2017 Probing single processive molecular motors with high-speed optical tweezers and fluorescence microscopy
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Abstract
Here we present development of optical techniques for the study of single processive myosin motors based on the combination of high-speed optical tweezers force spectroscopy and single molecule fluorescence imaging. Ultrafast force-clamp spectroscopy1 is applied to study the dependence of single chemo-mechanical steps of processive myosin motors on the applied load. On the other hand, single molecule localization through FIONA (Fluorescence Imaging with One Nanometer Accuracy)2, 3 is applied to in vitro motility assay to measure parameters such as the runlength, velocity and step size of single myosin V motors, labeled with Quantum Dots, under unloaded conditions.
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L. Gardini, F. S. Pavone, M. Capitanio, "Probing single processive molecular motors with high-speed optical tweezers and fluorescence microscopy", Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 1007111 (21 February 2017); doi: 10.1117/12.2256564; https://doi.org/10.1117/12.2256564
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