17 February 2017 Detection of lipopolysaccharides in serum using a waveguide-based optical biosensor
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Direct ultra-sensitive detection of pathogen biomarkers in blood could provide a universal strategy for diagnosis of bacterial infections, which remain a leading cause of morbidity and mortality in many areas of the world. Many factors complicate diagnosis, including the presence of multiple co-infections in a given patient, and lack of infrastructure in rural settings. In some pediatric patients, such as those in areas with poor resources, an additional challenge exists with low sample volumes due to age and other health factors such as anemia and dehydration. Our team is working on developing novel diagnostic assays, with a waveguide-based biosensor platform, to rapidly and specifically identify pathogen biomarkers from small samples of serum or plasma, allowing for the timely and sensitive diagnosis of infection at the point of care. In addition to the platform, we have developed novel membrane insertion and lipoprotein capture assay methods, to capture lipidated pathogen biomarkers in aqueous blood, by virtue of their interactions with host lipoprotein carriers. Herein, we demonstrate our efforts to adapt the lipoprotein capture assay for the detection of small concentrations of pathogen-secreted lipopolysaccharides in aqueous blood, with the ultimate aim of diagnosing Gram-negative infections effectively.
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Aneesa Noormohamed, Aneesa Noormohamed, Loreen R. Stromberg, Loreen R. Stromberg, Aaron S. Anderson, Aaron S. Anderson, Zachary Karim, Zachary Karim, Priya Dighe, Priya Dighe, Prakasha Kempaiah, Prakasha Kempaiah, John M. Ong'echa, John M. Ong'echa, Douglas J. Perkins, Douglas J. Perkins, Norman Doggett, Norman Doggett, Benjamin McMahon, Benjamin McMahon, Harshini Mukundan, Harshini Mukundan, } "Detection of lipopolysaccharides in serum using a waveguide-based optical biosensor", Proc. SPIE 10072, Optical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics, 100720A (17 February 2017); doi: 10.1117/12.2253506; https://doi.org/10.1117/12.2253506

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