Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain; Nicholas A. Vickers; Bennett B. Goldberg; Thomas Bifano; Jerome Mertz

doi:10.1117/12.2271502

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21 February 2017 Extended depth-of-field microscopy with a high-speed deformable mirror

William J. Shain, Nicholas A. Vickers, Bennett B. Goldberg, Thomas Bifano, Jerome Mertz

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Proceedings Volume 10073, Adaptive Optics and Wavefront Control for Biological Systems III; 100730E (2017) https://doi.org/10.1117/12.2271502
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

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William J. Shain, Nicholas A. Vickers, Bennett B. Goldberg, Thomas Bifano, and Jerome Mertz "Extended depth-of-field microscopy with a high-speed deformable mirror", Proc. SPIE 10073, Adaptive Optics and Wavefront Control for Biological Systems III, 100730E (21 February 2017); https://doi.org/10.1117/12.2271502

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