21 February 2017 Extended depth-of-field microscopy with a high-speed deformable mirror
Author Affiliations +
Abstract
Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.
Conference Presentation
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
William J. Shain, William J. Shain, Nicholas A. Vickers, Nicholas A. Vickers, Bennett B. Goldberg, Bennett B. Goldberg, Thomas Bifano, Thomas Bifano, Jerome Mertz, Jerome Mertz, } "Extended depth-of-field microscopy with a high-speed deformable mirror", Proc. SPIE 10073, Adaptive Optics and Wavefront Control for Biological Systems III, 100730E (21 February 2017); doi: 10.1117/12.2271502; https://doi.org/10.1117/12.2271502
PROCEEDINGS
7 PAGES + PRESENTATION

SHARE
Back to Top