21 February 2017 A model for quantifying contrast enhancement in optical coherence tomography (OCT)
Author Affiliations +
Proceedings Volume 10074, Quantitative Phase Imaging III; 100740J (2017) https://doi.org/10.1117/12.2254498
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
We have developed a model to accurately quantify the signals produced by exogenous scattering agents used for contrast-enhanced Optical Coherence Tomography (OCT). This model predicts distinct concentration-dependent signal trends that arise from the underlying physics of coherence-based detection. Accordingly, we show that real scattering particles can be described as simplified ideal scatterers with modified scattering intensity and concentration. The relation between OCT signal and particle concentration is approximately linear at concentrations lower than 0.8 particles per imaging voxel. However, at higher concentrations, interference effects cause signal to increase with a square root dependence on the number of particles within a voxel. Finally, high particle concentrations cause enough light attenuation to saturate the detected signal. Predictions were validated by comparison with measured OCT signals from gold nanorods (GNRs) prepared in water at concentrations ranging over five orders of magnitude (50 fM to 5nM). In addition, we validated that our model accurately predicts the signal responses of GNRs in highly heterogeneous scattering environments including whole blood and living animals. By enabling particle quantification, this work provides a valuable tool for current and future contrast-enhanced in vivo OCT studies. More generally, the model described herein may be applied for detected signals in other modalities that rely on coherence-based detection or are susceptible to interference effects, most notably medical ultrasound. Thus, our model may enable quantitative interpretation of ultrasound contrast agents including gas-filled microbubbles.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yonatan Winetraub, Yonatan Winetraub, Elliott SoRelle, Elliott SoRelle, Orly Liba, Orly Liba, Adam de la Zerda, Adam de la Zerda, } "A model for quantifying contrast enhancement in optical coherence tomography (OCT)", Proc. SPIE 10074, Quantitative Phase Imaging III, 100740J (21 February 2017); doi: 10.1117/12.2254498; https://doi.org/10.1117/12.2254498
PROCEEDINGS
7 PAGES


SHARE
Back to Top