Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup

Nir A. Turko; Michael Isbach; Steffi Ketelhut; Burkhard Greve; Jürgen Schnekenburger; Natan T. Shaked; Björn Kemper

doi:10.1117/12.2252873

21 February 2017 Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup

Nir A. Turko, Michael Isbach, Steffi Ketelhut, Burkhard Greve, Jürgen Schnekenburger, Natan T. Shaked, Björn Kemper

Author Affiliations +

Proceedings Volume 10074, Quantitative Phase Imaging III; 100740Q (2017) https://doi.org/10.1117/12.2252873
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We explored photothermal quantitative phase imaging (PTQPI) of living cells with functionalized nanoparticles (NPs) utilizing a cost-efficient setup based on a cell culture microscope. The excitation light was modulated by a mechanical chopper wheel with low frequencies. Quantitative phase imaging (QPI) was performed with Michelson interferometer-based off-axis digital holographic microscopy and a standard industrial camera. We present results from PTQPI observations on breast cancer cells that were incubated with functionalized gold NPs binding to the epidermal growth factor receptor. Moreover, QPI was used to quantify the impact of the NPs and the low frequency light excitation on cell morphology and viability.

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Nir A. Turko, Michael Isbach, Steffi Ketelhut, Burkhard Greve, Jürgen Schnekenburger, Natan T. Shaked, and Björn Kemper "Photothermal quantitative phase imaging of living cells with nanoparticles utilizing a cost-efficient setup", Proc. SPIE 10074, Quantitative Phase Imaging III, 100740Q (21 February 2017); https://doi.org/10.1117/12.2252873

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