21 February 2017 Low cost label-free live cell imaging for biological samples
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Proceedings Volume 10074, Quantitative Phase Imaging III; 100741I (2017) https://doi.org/10.1117/12.2246680
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
This paper reports the progress to develop a practical phase measuring microscope offering new capabilities in terms of phase measurement accuracy and quantification of cell:cell interactions over the longer term. A novel, low cost phase interference microscope for imaging live cells (label-free) is described. The method combines the Zernike phase contrast approach with a dual mirror design to enable phase modulation between the scattered and un-scattered optical fields. Two designs are proposed and demonstrated, one of which retains the common path nature of Zernike’s original microscopy concept. In both setups the phase shift is simple to control via a piezoelectric driven mirror in the back focal plane of the imaging system. The approach is significantly cheaper to implement than those based on spatial light modulators (SLM) at approximately 20% of the cost. A quantitative assessment of the performance of a set of phase shifting algorithms is also presented, specifically with regard to broad bandwidth illumination in phase contrast microscopy. The simulation results show that the phase measurement accuracy is strongly dependent on the algorithm selected and the optical path difference in the sample.
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C. Seniya, C. Seniya, C. E. Towers, C. E. Towers, D. P. Towers, D. P. Towers, } "Low cost label-free live cell imaging for biological samples", Proc. SPIE 10074, Quantitative Phase Imaging III, 100741I (21 February 2017); doi: 10.1117/12.2246680; https://doi.org/10.1117/12.2246680
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