22 February 2017 Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy
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Abstract
Propagation of action potentials arises on millisecond timescales, suggesting the need for advancement of methods capable of commensurate volume rendering for in vivo brain mapping. In practice, beam-scanning multiphoton microscopy is widely used to probe brain function, striking a balance between simplicity and penetration depth. However, conventional beam-scanning platforms generally do not provide access to full volume renderings at the speeds necessary to map propagation of action potentials. By combining a sparse sampling strategy based on Lissajous trajectory microscopy in combination with temporal multiplexing for simultaneous imaging of multiple focal planes, whole volumes of cells are potentially accessible each millisecond.
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Andreas C. Geiger, Justin A. Newman, Suhas Sreehari, Shane Z. Sullivan, Charles A. Bouman, Garth J. Simpson, "Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy", Proc. SPIE 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II, 1007606 (22 February 2017); doi: 10.1117/12.2253514; https://doi.org/10.1117/12.2253514
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