22 February 2017 The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging
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Abstract
We have simulated the effects of the number of bits and the sampling rate of a digitizer on the performance of lifetime measurements. We found that the number of bits of a digitizer is important to obtain certain accuracy in lifetime measurement. There exists a certain critical sampling frequency of a digitizer required to separate a certain lifetime differences in a double exponentially decaying intensity profile. We did these simulations by using Monte Carlo simulations with least-square curve fitting algorithms.
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Won Sang Hwang, Dong Eun Kim, Jun Woo Kim, Youn Young Ji, Byung Hwy So, Dug Young Kim, "The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging", Proc. SPIE 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II, 100761H (22 February 2017); doi: 10.1117/12.2250730; https://doi.org/10.1117/12.2250730
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