Time multiplexing super-resolution nanoscopy based on the Brownian motion of gold nanoparticles

Tali Ilovitsh; Asaf Ilovitsh; Omer Wagner; Zeev Zalevsky

doi:10.1117/12.2250277

22 February 2017 Time multiplexing super-resolution nanoscopy based on the Brownian motion of gold nanoparticles

Tali Ilovitsh, Asaf Ilovitsh, Omer Wagner, Zeev Zalevsky

Author Affiliations +

Proceedings Volume 10077, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV; 100770R (2017) https://doi.org/10.1117/12.2250277
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Super-resolution localization microscopy can overcome the diffraction limit and achieve a tens of order improvement in resolution. It requires labeling the sample with fluorescent probes followed with their repeated cycles of activation and photobleaching. This work presents an alternative approach that is free from direct labeling and does not require the activation and photobleaching cycles. Fluorescently labeled gold nanoparticles in a solution are distributed on top of the sample. The nanoparticles move in a random Brownian motion, and interact with the sample. By obscuring different areas in the sample, the nanoparticles encode the sub-wavelength features. A sequence of images of the sample is captured and decoded by digital post processing to create the super-resolution image. The achievable resolution is limited by the additive noise and the size of the nanoparticles. Regular nanoparticles with diameter smaller than 100nm are barely seen in a conventional bright field microscope, thus fluorescently labeled gold nanoparticles were used, with proper

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Tali Ilovitsh, Tali Ilovitsh, Asaf Ilovitsh, Asaf Ilovitsh, Omer Wagner, Omer Wagner, Zeev Zalevsky, Zeev Zalevsky, } "Time multiplexing super-resolution nanoscopy based on the Brownian motion of gold nanoparticles", Proc. SPIE 10077, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV, 100770R (22 February 2017); doi: 10.1117/12.2250277; https://doi.org/10.1117/12.2250277

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