21 February 2017 Silica passivated conjugated polymer nanoparticles for biological imaging applications
Author Affiliations +
Abstract
Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE-1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.
Conference Presentation
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Struan Bourke, Struan Bourke, Laura Urbano, Laura Urbano, Antoni Olona, Antoni Olona, Ferran Valderrama, Ferran Valderrama, Lea Ann Dailey, Lea Ann Dailey, Mark A. Green, Mark A. Green, } "Silica passivated conjugated polymer nanoparticles for biological imaging applications", Proc. SPIE 10079, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX, 100790A (21 February 2017); doi: 10.1117/12.2252035; https://doi.org/10.1117/12.2252035
PROCEEDINGS
13 PAGES + PRESENTATION

SHARE
Back to Top