Time-resolved SERS for characterizing extracellular vesicles

Tatu Rojalin; Heikki Saari; Petter Somersalo; Saara Laitinen; Mikko Turunen; Tapani Viitala; Sebastian Wachsmann-Hogiu; Zachary J. Smith; Marjo Yliperttula

doi:10.1117/12.2271865

3 April 2017 Time-resolved SERS for characterizing extracellular vesicles

Tatu Rojalin, Heikki Saari, Petter Somersalo, Saara Laitinen, Mikko Turunen, Tapani Viitala, Sebastian Wachsmann-Hogiu, Zachary J. Smith, Marjo Yliperttula

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Proceedings Volume 10080, Plasmonics in Biology and Medicine XIV; 100800U (2017) https://doi.org/10.1117/12.2271865
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The aim of this work is to develop a platform for characterizing extracellular vesicles (EV) by using gold-polymer nanopillar SERS arrays simultaneously circumventing the photoluminescence-related disadvantages of Raman with a time-resolved approach. EVs are rich of biochemical information reporting of, for example, diseased state of the biological system. Currently, straightforward, label-free and fast EV characterization methods with low sample consumption are warranted. In this study, SERS spectra of red blood cell and platelet derived EVs were successfully measured and their biochemical contents analyzed using multivariate data analysis techniques. The developed platform could be conveniently used for EV analytics in general.

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Tatu Rojalin, Heikki Saari, Petter Somersalo, Saara Laitinen, Mikko Turunen, Tapani Viitala, Sebastian Wachsmann-Hogiu, Zachary J. Smith, and Marjo Yliperttula "Time-resolved SERS for characterizing extracellular vesicles", Proc. SPIE 10080, Plasmonics in Biology and Medicine XIV, 100800U (3 April 2017); https://doi.org/10.1117/12.2271865

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