In this work, we propose integration of FLM with micro-injection moulding (μIM) as a novel route towards the cost-effective and flexible manufacturing of polymeric Lab-on-a-Chip (LOC) devices. In particular, we have fabricated and assembled a polymethylmethacrylate (PMMA) microfluidic optical stretcher by exploiting firstly FLM to manufacture a metallic mould prototype with reconfigurable inserts. Afterwards, such mould was employed for the production, through μIM, of the two PMMA thin plates composing the device. The microchannel with reservoirs and lodgings for the optical fibers delivering the laser radiation for cell trapping were reproduced on one plate, while the other included access holes to the channel. The device was assembled by direct fs-laser welding, ensuring sealing of the channel and avoiding thermal deformation and/or contamination.
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Annalisa Volpe, Antonio Ancona, Gianluca Trotta, Rebeca Martínez Vázquez, Irene Fassi, Roberto Osellame, "Fabrication and assembling of a microfluidic optical stretcher polymeric chip combining femtosecond laser and micro injection molding technologies," Proc. SPIE 10092, Laser-based Micro- and Nanoprocessing XI, 100920F (17 February 2017);