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20 February 2017 Round Robin test on bio-imaging transfer standard for 3D optical profilers
A. Nolvi, T. Viitala, A. García Pérez, N. Sandler, E. Hæggström, C. Bermudez, R. Artigas, I. Kassamakov
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Proceedings Volume 10110, Photonic Instrumentation Engineering IV; 101100M (2017) https://doi.org/10.1117/12.2250261
Event: SPIE OPTO, 2017, San Francisco, California, United States
Abstract
A stair case height Bio-Transfer-Standard (BTS), developed and produced at the University of Helsinki (UH), was measured in two laboratories. The Round Robin test aims to determine whether BTS works with different optical profilers in different laboratories. First the artefact was measured at UH using a custom-built Scanning White Light Interferometer. Then BTS was measured at Sensofar-Tech, S.L. using an S-neox-type interferometer working either in Phase Shifting Interferometry mode or in Imaging Confocal Microscopy mode. To remove the influence of system calibration, a method featuring sample shifting and measurement subtraction was used. The BTS features eight lipid bilayer steps that each are 4.6 ± 0.1 nm tall on average. All 30 measurements done by four different operators at the two laboratories agree to within 0.1 nm which agrees with theoretical estimates and with measurements done using a surface plasmon resonance technique. The Round Robin results show the applicability of the newly developed bio-imaging transfer standard for calibrating 3D optical profilers.
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A. Nolvi, T. Viitala, A. García Pérez, N. Sandler, E. Hæggström, C. Bermudez, R. Artigas, and I. Kassamakov "Round Robin test on bio-imaging transfer standard for 3D optical profilers", Proc. SPIE 10110, Photonic Instrumentation Engineering IV, 101100M (20 February 2017); https://doi.org/10.1117/12.2250261
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KEYWORDS
Calibration
Confocal microscopy
Objectives
Standards development
Microscopes
Interferometers
3D metrology
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