27 February 2017 Confining Brownian motion of single nanoparticles in an ABELtrap
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Proceedings Volume 10120, Complex Light and Optical Forces XI; 1012017 (2017) https://doi.org/10.1117/12.2250550
Event: SPIE OPTO, 2017, San Francisco, California, United States
Abstract
Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a confocal laser focus. We present an Anti-Brownian ELectrokinetic trap1–7 (pioneered by A. E. Cohen and W. E. Moerner) to hold nanoparticles and individual FoF1-ATP synthase proteins in solution. We are interested in the conformational dynamics of this membrane-bound rotary motor protein that we monitor using single-molecule FRET. The ABELtrap is an active feedback system cancelling the nano-object’s Brownian motion by applying an electric field. We show how the induced electrokinetic forces confine the motion of nanoparticles and proteoliposomes to the centre of the trap.
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Maria Dienerowitz, Maria Dienerowitz, Thomas Heitkamp, Thomas Heitkamp, Thomas Gottschall, Thomas Gottschall, Jens Limpert, Jens Limpert, Michael Börsch, Michael Börsch, } "Confining Brownian motion of single nanoparticles in an ABELtrap", Proc. SPIE 10120, Complex Light and Optical Forces XI, 1012017 (27 February 2017); doi: 10.1117/12.2250550; https://doi.org/10.1117/12.2250550
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