Translator Disclaimer
27 February 2017 Optical cell sorting with multiple imaging modalities
Author Affiliations +
Proceedings Volume 10120, Complex Light and Optical Forces XI; 1012018 (2017)
Event: SPIE OPTO, 2017, San Francisco, California, United States
Early detection of diseases can save lives. Hence, there is emphasis in sorting rare disease-indicating cells within small dilute quantities such as in the confines of lab-on-a-chip devices. However, before diseased cells can be studied in isolation, it is necessary to identify them against normal healthy cells. With the richness of visual information, a lot of microscopy techniques have been developed and have been crucial in biological studies. To utilize their complementary advantages we adopt both fluorescence and brightfield imaging in our optical cell sorter. Brightfield imaging has the advantage of being non-invasive, thus maintaining cell viability. Fluorescence imaging, on the other hand, takes advantages of the chemical specificity of fluorescence markers and can validate machine vision results from brightfield images. Visually identified cells are sorted using optical manipulation techniques. Scattering forces from beams actuated via efficient phase-only efficient modulation has been adopted. This has lowered the required power for sorting cells to a tenth of our previous approach, and also makes the cell sorter safer for use in clinical settings. With the versatility of dynamically programmable phase spatial light modulators, a plurality of light shaping techniques, including hybrid approaches, can be utilized in cell sorting.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Andrew Bañas, Caro Carrissemoux, Mark Villangca, Darwin Palima, and Jesper Glückstad "Optical cell sorting with multiple imaging modalities", Proc. SPIE 10120, Complex Light and Optical Forces XI, 1012018 (27 February 2017);


Cell sorting using efficient light shaping approaches
Proceedings of SPIE (March 03 2016)
Predictive visual tracking
Proceedings of SPIE (August 05 1993)
Eggshell defects detection based on color processing
Proceedings of SPIE (March 20 2000)
Mobile cosmetics advisor: an imaging based mobile service
Proceedings of SPIE (January 27 2010)

Back to Top