Vortex self-imaging experiments on aberration insensitive localization of micro-objects

M. Baránek; P. Bouchal; Z. Bouchal

doi:10.1117/12.2264356

23 December 2016 Vortex self-imaging experiments on aberration insensitive localization of micro-objects

M. Baránek, P. Bouchal, Z. Bouchal

Proceedings Volume 10142, 20th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics; 101420N (2016) https://doi.org/10.1117/12.2264356
Event: 20th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 2016, Jasna, Slovakia

Abstract

In recent years, optical microscopy has been enriched by a wide range of modern techniques enabling exploration of volume samples. One of the preferred ways for reaching a depth estimation required in three-dimensional (3D) imaging is based on utilization of optical systems working with a point spread function (PSF) that rotates under defocusing. Here, the method of axial localization of microparticles is examined that is based on the evaluation of the PSF rotation caused by interference of the vortex nondiffracting beams (VNBs). For generation of the VNBs, a special complex mask is used, modulating both amplitude and phase of the spatial spectrum of the specimen. The main attention is focused on examination of the optical performance of the method and analysis of the effects that occur, when the mask is implemented using a spatial light modulator (SLM).

Citation Download Citation

M. Baránek, P. Bouchal, and Z. Bouchal "Vortex self-imaging experiments on aberration insensitive localization of micro-objects", Proc. SPIE 10142, 20th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 101420N (23 December 2016); https://doi.org/10.1117/12.2264356

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