Fabrication MoS2 biosensor to detect lower-concentrated area of biological molecules (Conference Presentation)

Erika Yang; Byunghoon Ryu; Hongsuk Nam; Xiaogan Liang

doi:10.1117/12.2258197

25 May 2017 Fabrication of MoS₂ biosensor to detect lower-concentrated area of biological molecules(Conference Presentation)

Erika Yang, Byunghoon Ryu, Hongsuk Nam, Xiaogan Liang

Author Affiliations +

Proceedings Volume 10146, Advances in Patterning Materials and Processes XXXIV; 101461E (2017) https://doi.org/10.1117/12.2258197
Event: SPIE Advanced Lithography, 2017, San Jose, California, United States

Abstract

Two dimensional layered transition metal dichalcogenides (TMDC) materials have the growing potential to upstage graphene in the next generation of biosensors in detecting lower-concentrated areas of biomolecules. The current gold-standard detection method is the enzyme-linked immunosorbent assay (ELISA), an immunological assay technique that makes use of an enzyme bonded to a particular antibody or antigen. However, this technique is not only bulky, labor-intensive, and time extensive, but more importantly, the ELISA has relatively low detection limits of only 600 femtomolar (fM). In this work, for the first time, we present a novel flexible, sensitive MoS2 (molybdenum disulfide) biosensor, as shown in Figure 1, composed of few-layer of MoS2 as the channel material, and flexible polyimide as the substrate. In order to nano-fabricate this flexible biosensor, we mechanically transferred a few layers of MoS2 onto the flexible substrate polyimide and photolithography to create a patterning on the surface, and as a result, we were able to create a transistor that used MoS2 as its conductance channel. We successfully fabricated this MoS2 biosensor onto a flexible polyimide substrate. Furthermore, the fabricated flexible MoS2 biosensor can be utilized for quantifying the time-dependent reaction kinetics of streptavidin-biotin binding. Figure 2 shows the transfer characteristics of flexible MoS2 biosensors measured under different concentrations of streptavidin. The flexible MoS2 biosensor could illustrate a faster detection time in matters of minutes, and higher sensitivity with detection limits as low as 10 fM. Time versus equilibrium constants will be presented in details.

Conference Presentation

Citation Download Citation

Erika Yang, Byunghoon Ryu, Hongsuk Nam, and Xiaogan Liang "Fabrication of MoS₂ biosensor to detect lower-concentrated area of biological molecules(Conference Presentation)", Proc. SPIE 10146, Advances in Patterning Materials and Processes XXXIV, 101461E (25 May 2017); https://doi.org/10.1117/12.2258197

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