11 November 2016 Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis
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Proceedings Volume 10150, Second International Seminar on Photonics, Optics, and Its Applications (ISPhOA 2016); 1015016 (2016) https://doi.org/10.1117/12.2248461
Event: Second International Seminar on Photonics, Optics, and Its Applications (ISPhOA 2016), 2016, Bali, Indonesia
Abstract
Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm-1, 1224 cm-1, 1375 cm-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.
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Surekha Barkur, Aseefhali Bankapur, Santhosh Chidangil, "Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis", Proc. SPIE 10150, Second International Seminar on Photonics, Optics, and Its Applications (ISPhOA 2016), 1015016 (11 November 2016); doi: 10.1117/12.2248461; https://doi.org/10.1117/12.2248461
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