19 October 2016 Novel glucose fiber sensor combining ThFBG with GOD
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Proceedings Volume 10155, Optical Measurement Technology and Instrumentation; 101550L (2016) https://doi.org/10.1117/12.2244494
Event: International Symposium on Optoelectronic Technology and Application 2016, 2016, Beijing, China
Abstract
We propose a novel glucose fiber optic sensor combining a thinned cladding fiber Bragg grating (ThFBG) with glucose oxidase (GOD). By immobilizing GOD on the surface of a ThFBG, the fabricated sensor can obtain a high specificity to glucose. Because of the evanescent field, the sensor is very sensitive to the ambient refractive index change arising from the catalytic reaction between glucose and GOD. A four-level fiber model was simulated and verified the precision of the sensing principle. Two methods, glutaraldehyde crosslinking method (GCM) and 3-aminopropyl triethoxysilane covalent coupling method (ATCCM), were experimentally utilized to immobilize GOD. And sensor fabricated with the method ATCCM shows a measurement range of 0-0.82 mg/mL which is better than the sensor fabricated with the method GCM with measurement range of 0-0.67 mg/mL under the same condition. By using ATCCM to immobilize GOD with different concentrations, three sensors were fabricated and used for glucose measurement by monitoring the Bragg wavelength (λb) shifts, the results indicate a good linear relationship between wavelength shift and glucose concentration within a specific range, and the measurement range increases as GOD concentration increases. The highest sensitivity of sensor reaches up to 0.0549 nm/(mg.mL-1). The proposed sensor has distinct advantages in sensing structure, cost and specificity.
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Mengmeng Li, Mengmeng Li, Ciming Zhou, Ciming Zhou, Dian Fan, Dian Fan, Yiwen Ou, Yiwen Ou, } "Novel glucose fiber sensor combining ThFBG with GOD", Proc. SPIE 10155, Optical Measurement Technology and Instrumentation, 101550L (19 October 2016); doi: 10.1117/12.2244494; https://doi.org/10.1117/12.2244494
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