We demonstrated that the high spatial resolution absorption contrast imaging of the crystal of vitamin B9 having absorption at UV wavelengths. The absorption wavelength matches with the wavelength of the emission of the fluorescent thin film we deposited. The fine crystal structure was imaged beyond the optical diffraction limit. The image contrast corresponded with the thickness of the crystal measured with an atomic force microscope (AFM). The illumination light is absorbed with the vitamin B9 crystal and the intensity of the transmitted light depends on the thickness of the vitamin B9 crystal. The EXA microscope is useful for analysis of growth of a crystal, bio-imaging, and so on.
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Wataru Inami, Masahiro Fukuta, Yoshimasa Kawata, Susumu Terakawa, "High spatial resolution absorption contrast imaging with electron-beam excitation assisted optical microscope," Proc. SPIE 10176, Asia-Pacific Conference on Fundamental Problems of Opto- and Microelectronics, 1017617 (14 December 2016);