1 May 2017 Phage-based biomolecular filter for the capture of bacterial pathogens in liquid streams
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Abstract
This paper investigates a phage-based biomolecular filter that enables the evaluation of large volumes of liquids for the presence of small quantities of bacterial pathogens. The filter is a planar arrangement of phage-coated, strip-shaped magnetoelastic (ME) biosensors (4 mm × 0.8 mm × 0.03 mm), magnetically coupled to a filter frame structure, through which a liquid of interest flows. This "phage filter" is designed to capture specific bacterial pathogens and allow non-specific debris to pass, eliminating the common clogging issue in conventional bead filters. ANSYS Maxwell was used to simulate the magnetic field pattern required to hold ME biosensors densely and to optimize the frame design. Based on the simulation results, a phage filter structure was constructed, and a proof-in-concept experiment was conducted where a Salmonella solution of known concentration were passed through the filter, and the number of captured Salmonella was quantified by plate counting.
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Songtao Du, I-Hsuan Chen, Shin Horikawa, Xu Lu, Yuzhe Liu, Howard C. Wikle, Sang Jin Suh, Bryan A. Chin, "Phage-based biomolecular filter for the capture of bacterial pathogens in liquid streams", Proc. SPIE 10217, Sensing for Agriculture and Food Quality and Safety IX, 1021702 (1 May 2017); doi: 10.1117/12.2262347; https://doi.org/10.1117/12.2262347
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