1 May 2017 Detecting benzoyl peroxide in wheat flour by line-scan macro-scale Raman chemical imaging
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Abstract
Excessive use of benzoyl peroxide (BPO, a bleaching agent) in wheat flour can destroy flour nutrients and cause diseases to consumers. A macro-scale Raman chemical imaging method was developed for direct detection of BPO mixed in the wheat flour. A 785 nm line laser was used in a line-scan Hyperspectral Raman imaging system. Raman images were collected from wheat flour mixed with BPO at eight concentrations (w/w) from 50 to 6,400 ppm. A sample holder (150×100×2 mm3) was used to present a thin layer (2 mm thick) of the powdered sample for image acquisition. A baseline correction method was used to correct the fluctuating fluorescence signals from the wheat flour. To isolate BPO particles from the flour background, a simple thresholding method was applied to the single-band fluorescence-free images at a unique Raman peak wavenumber (i.e., 1001 cm−1) preselected for the BPO detection. Chemical images were created to detect and map the BPO particles. Limit of detection for the BPO was estimated in the order of 50 ppm, which is on the same level with regulatory standards.
Conference Presentation
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Jianwei Qin, Jianwei Qin, Moon S. Kim, Moon S. Kim, Kuanglin Chao, Kuanglin Chao, Maria Gonzalez, Maria Gonzalez, Byoung-Kwan Cho, Byoung-Kwan Cho, } "Detecting benzoyl peroxide in wheat flour by line-scan macro-scale Raman chemical imaging", Proc. SPIE 10217, Sensing for Agriculture and Food Quality and Safety IX, 1021707 (1 May 2017); doi: 10.1117/12.2262660; https://doi.org/10.1117/12.2262660
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