Computational microscopy: illumination coding and nonlinear optimization enables gigapixel 3D phase imaging

Lei Tian; Laura Waller

doi:10.1117/12.2264063

10 May 2017 Computational microscopy: illumination coding and nonlinear optimization enables gigapixel 3D phase imaging

Lei Tian, Laura Waller

Proceedings Volume 10219, Three-Dimensional Imaging, Visualization, and Display 2017; 102190N (2017) https://doi.org/10.1117/12.2264063
Event: SPIE Commercial + Scientific Sensing and Imaging, 2017, Anaheim, CA, United States

Abstract

Microscope lenses can have either large field of view (FOV) or high resolution, not both. Computational microscopy based on illumination coding circumvents this limit by fusing images from different illumination angles using nonlinear optimization algorithms. The result is a Gigapixel-scale image having both wide FOV and high resolution. We demonstrate an experimentally robust reconstruction algorithm based on a 2nd order quasi-Newton's method, combined with a novel phase initialization scheme. To further extend the Gigapixel imaging capability to 3D, we develop a reconstruction method to process the 4D light field measurements from sequential illumination scanning. The algorithm is based on a 'multislice' forward model that incorporates both 3D phase and diffraction effects, as well as multiple forward scatterings. To solve the inverse problem, an iterative update procedure that combines both phase retrieval and 'error back-propagation' is developed. To avoid local minimum solutions, we further develop a novel physical model-based initialization technique that accounts for both the geometric-optic and 1st order phase effects. The result is robust reconstructions of Gigapixel 3D phase images having both wide FOV and super resolution in all three dimensions. Experimental results from an LED array microscope were demonstrated.

Conference Presentation

Citation Download Citation

Lei Tian and Laura Waller "Computational microscopy: illumination coding and nonlinear optimization enables gigapixel 3D phase imaging", Proc. SPIE 10219, Three-Dimensional Imaging, Visualization, and Display 2017, 102190N (10 May 2017); https://doi.org/10.1117/12.2264063

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