UV-SPR biosensor for biomolecular interaction studies

F. A. Geiss; S. Fossati; I. Khan; N. Gisbert Quilis; W. Knoll; J. Dostalek

doi:10.1117/12.2265683

16 May 2017 UV-SPR biosensor for biomolecular interaction studies

F. A. Geiss, S. Fossati, I. Khan, N. Gisbert Quilis , W. Knoll, J. Dostalek

Author Affiliations +

Proceedings Volume 10231, Optical Sensors 2017; 1023107 (2017) https://doi.org/10.1117/12.2265683
Event: SPIE Optics + Optoelectronics, 2017, Prague, Czech Republic

Abstract

UV surface plasmon resonance (SPR) for direct in situ detection of protein binding events is reported. A crossed relief aluminum grating was employed for diffraction coupling to surface plasmons as an alternative to more commonly used attenuated total reflection method. Wavelength interrogation of SPR was carried out by using transmission measurements in order to probe odorant-binding protein 14 (OBP14) of the honey bee (Apis mellifera). The native oxide layer on the top of an aluminum grating sensor chip allows for covalent coupling of protein molecules by using regular silane-based linkers. The probing of bound OBP14 protein at UV with confined field of surface plasmons holds potential for further studies of interaction with recently developed artificial fluorescent odorants.

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F. A. Geiss, S. Fossati, I. Khan, N. Gisbert Quilis , W. Knoll, and J. Dostalek "UV-SPR biosensor for biomolecular interaction studies", Proc. SPIE 10231, Optical Sensors 2017, 1023107 (16 May 2017); https://doi.org/10.1117/12.2265683

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