13 June 2017 Development of a lab-on-chip electrochemical immunosensor for detection of Polycyclic Aromatic Hydrocarbons (PAH) in environmental water
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Abstract
The work described in this manuscript focuses on how the integration of immunoassay techniques in combination with electrochemical detection can provide a portable and very accurate solution for detection of water pollutants that are detrimental for human health. In particular, we focus our work on the quantification of polycyclic aromatic hydrocarbons (PAHs) in polluted water. Our integrative approach facilitates a real-time detection of this family of organic compounds, by reducing the time of analysis to less than one hour. Additionally, the use of a lab-on-a-chip platform delivers a portable solution that could be used in situ.

Optimization of a displacement assay that investigates the presence and concentration of Benzo[a]pyrene in water, allows with the miniaturization of the standard ELISA format into a highly accurate system that provides fast results. The limits of detection obtained are comparable to those of available state-of-the art tools, and achieve the values set by European Drinking Water Directive, 0.10ng/l, as the limit for PAHs in drinking water.
Conference Presentation
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Shifa Felemban, Shifa Felemban, Patricia Vazquez, Patricia Vazquez, Jan Dehnert, Jan Dehnert, Vadim Goridko, Vadim Goridko, Maria Tijero, Maria Tijero, Eric Moore, Eric Moore, } "Development of a lab-on-chip electrochemical immunosensor for detection of Polycyclic Aromatic Hydrocarbons (PAH) in environmental water", Proc. SPIE 10246, Smart Sensors, Actuators, and MEMS VIII, 102460B (13 June 2017); doi: 10.1117/12.2265011; https://doi.org/10.1117/12.2265011
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