30 May 2017 Inkjet-printed selective microfluidic biosensor using CNTs functionalized by cytochrome P450 enzyme
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Abstract
An additive manufacturing concept, consisting of 3D photopolymer printing and Ag nanoparticle printing, was investigated for the construction of a microfluidic biosensor based on immobilized cytochrome P450 enzyme. An acylate-type microfluidic chamber composed of two parts, i.e. chamber-housing and chamber-lid was printed with a polyjet 3D printer. A 3-electrode sensor structure was inkjet-printed on the lid using a combination of Ag and graphene printing. The working electrode was covered with carbon nanotubes by drop-casting and immobilized with cytochrome P450 2D6 enzyme. The microfluidic sensor shows a significant response to a test xenobiotic, i.e. dextromethorphan; the cyclic voltammetrical measurements show a corresponding oxidation peak at 0.4 V with around 5 μM detection limit.
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Matic Krivec, Matic Krivec, Raimund Leitner, Raimund Leitner, Florian Überall, Florian Überall, Johannes Hochleitner, Johannes Hochleitner, } "Inkjet-printed selective microfluidic biosensor using CNTs functionalized by cytochrome P450 enzyme", Proc. SPIE 10247, Bio-MEMS and Medical Microdevices III, 1024707 (30 May 2017); doi: 10.1117/12.2264095; https://doi.org/10.1117/12.2264095
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