Microfluidic system for monitoring of cardiac (H9C2) cell proliferation

A. Kobuszewska; P. Cwik; E. Jastrzebska; Z. Brzozka; M. Chudy; P. Renaud; A. Dybko

doi:10.1117/12.2266356

30 May 2017 Microfluidic system for monitoring of cardiac (H9C2) cell proliferation

A. Kobuszewska, P. Cwik, E. Jastrzebska, Z. Brzozka, M. Chudy, P. Renaud, A. Dybko

Proceedings Volume 10247, Bio-MEMS and Medical Microdevices III; 102470E (2017) https://doi.org/10.1117/12.2266356
Event: SPIE Microtechnologies, 2017, Barcelona, Spain

Abstract

The paper presents the application of electrical impedance spectroscopy (EIS) analysis for investigation of cardiac cell (H9C2 – rat cardiomyoblast) proliferation after verapamil hydrochloride exposure. For this purpose, two different PDMS/glass microsystems with circular microchamber and longitudinal microchannel integrated with Pt/Al electrodes were used. The microchambers were fabricated in PDMS using photolithography and replica moulding techniques. Pt/Al electrodes were fabricated on a 4-inch glass substrate using Physical Vapor Deposition (PVD). Solution of verapamil hydrochloride was continuously introduced into the microsystems with H9C2 cell culture (a flow rate of 1 μl/min) for 72 h. The impedance spectra were recorded from 100 Hz to 1 MHz. We confirmed that impedance spectroscopy can be used for non-invasive, label-free and real-time analysis of cardiac cells proliferation based on cells dielectric properties and biological structure.

Citation Download Citation

A. Kobuszewska, P. Cwik, E. Jastrzebska, Z. Brzozka, M. Chudy, P. Renaud, and A. Dybko "Microfluidic system for monitoring of cardiac (H9C2) cell proliferation", Proc. SPIE 10247, Bio-MEMS and Medical Microdevices III, 102470E (30 May 2017); https://doi.org/10.1117/12.2266356

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