Two-photon excitation microscopy with spatial light modulator

Naoya Matsumoto; Alu Konno; Takashi Inoue; Haruyoshi Toyoda; Toshiyuki Miwa; Kazuhiro Nakamura; Shigetoshi Okazaki

doi:10.1117/12.2269406

18 April 2017 Two-photon excitation microscopy with spatial light modulator

Naoya Matsumoto; Alu Konno; Takashi Inoue; Haruyoshi Toyoda; Toshiyuki Miwa; Kazuhiro Nakamura; Shigetoshi Okazaki

Author Affiliations +

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 1025105 (2017) https://doi.org/10.1117/12.2269406
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

We attempted to observe deep regions in biological samples through two-photon excitation microscopy adopting a spatial light modulator (SLM). The SLM is used for correcting spherical aberration (SA) caused by the refractive-index mismatch between the immersion medium and sample. In the observation of fluorescent beads in transparent epoxy resin, the fluorescence intensity from a scan with SA correction was 50 times that from a scan without SA correction. After that, we observed blood vessels in a mouse brain, which became transparent with a clearing agent.

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Naoya Matsumoto, Naoya Matsumoto, Alu Konno, Alu Konno, Takashi Inoue, Takashi Inoue, Haruyoshi Toyoda, Haruyoshi Toyoda, Toshiyuki Miwa, Toshiyuki Miwa, Kazuhiro Nakamura, Kazuhiro Nakamura, Shigetoshi Okazaki, Shigetoshi Okazaki, } "Two-photon excitation microscopy with spatial light modulator", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 1025105 (18 April 2017); doi: 10.1117/12.2269406; https://doi.org/10.1117/12.2269406

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