18 April 2017 Two-photon excitation microscopy with spatial light modulator
Author Affiliations +
Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 1025105 (2017) https://doi.org/10.1117/12.2269406
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan
We attempted to observe deep regions in biological samples through two-photon excitation microscopy adopting a spatial light modulator (SLM). The SLM is used for correcting spherical aberration (SA) caused by the refractive-index mismatch between the immersion medium and sample. In the observation of fluorescent beads in transparent epoxy resin, the fluorescence intensity from a scan with SA correction was 50 times that from a scan without SA correction. After that, we observed blood vessels in a mouse brain, which became transparent with a clearing agent.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Naoya Matsumoto, Naoya Matsumoto, Alu Konno, Alu Konno, Takashi Inoue, Takashi Inoue, Haruyoshi Toyoda, Haruyoshi Toyoda, Toshiyuki Miwa, Toshiyuki Miwa, Kazuhiro Nakamura, Kazuhiro Nakamura, Shigetoshi Okazaki, Shigetoshi Okazaki, } "Two-photon excitation microscopy with spatial light modulator", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 1025105 (18 April 2017); doi: 10.1117/12.2269406; https://doi.org/10.1117/12.2269406

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