Improvement of spatial and spectral resolution in Raman microscopy

Katsumasa Fujita

doi:10.1117/12.2275673

18 April 2017 Improvement of spatial and spectral resolution in Raman microscopy

Katsumasa Fujita

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 1025107 (2017) https://doi.org/10.1117/12.2275673
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

The classical limit of the spatial resolution, known as the diffraction limit, has been overcome by the recent development of super-resolution techniques. However, the main application of the technique is limited to fluorescence imaging. In our research, we have developed two techniques to improve the spatial resolution in Raman scattering imaging. We introduced the structured illumination to improve the spatial resolution in line-illumination Raman microscopy. We also utilized the saturation effect of coherent anti-Stokes Raman scattering to improve both spatial and spectral resolution simultaneously.

Citation Download Citation

Katsumasa Fujita "Improvement of spatial and spectral resolution in Raman microscopy", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 1025107 (18 April 2017); https://doi.org/10.1117/12.2275673

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