Microscopic video observation of capillary vessel systems using diffuse back lighting

Minako Sakai; Hiroki Arai; Toshiaki Iwai

doi:10.1117/12.2274907

18 April 2017 Microscopic video observation of capillary vessel systems using diffuse back lighting

Minako Sakai, Hiroki Arai, Toshiaki Iwai

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102510E (2017) https://doi.org/10.1117/12.2274907
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

We have been developing a simple and practical video microscopy system based on absorption spectra of biological substance to perform spectroscopic observation of living tissues. The diffuse backlighting effect is actively used in the developed system, which is generated by multiple light scattering in the tissue. It is demonstrated that the light specularly reflected from the skin surface can be completely suppressed in the microscopic observation and the biological activity of the capillary vessel systems distributed under the skin can be successfully observed. As a result, we can confirm the effectiveness of the video microscopy system using diffuse backlighting and the applicability of our developed system.

Citation Download Citation

Minako Sakai, Hiroki Arai, and Toshiaki Iwai "Microscopic video observation of capillary vessel systems using diffuse back lighting", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510E (18 April 2017); https://doi.org/10.1117/12.2274907

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