The optical heterogeneity of biological tissue imposes a major limitation to acquire detailed structural and functional information deep in the biological specimens using conventional microscopes. To restore optimal imaging performance, we developed an adaptive optical microscope based on direct wavefront sensing technique. This microscope can reliably measure and correct biological samples induced aberration. We demonstrated its performance and application in structural and functional brain imaging in various animal models, including fruit fly, zebrafish and mouse.
"Adaptive optical microscope for brain imaging in vivo", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510H (18 April 2017); doi: 10.1117/12.2274950; https://doi.org/10.1117/12.2274950