18 April 2017 Ultra-fast 3D scanning and holographic illumination in non-linear microscopy using acousto-optic deflectors
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Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102510L (2017); doi: 10.1117/12.2275143
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan
Abstract
Decoding of information in the brain requires the imaging of large neuronal networks using e.g. two-photon microscopy (TPM). Fast control of the focus in 3D can be achieved with phase shaping of the light beam using acoustooptic deflectors (AODs). However, beam shaping using AODs is not straightforward because of non-stationary of acousto-optic diffraction. Here, we demonstrated a new stable AOD-based phase modulator, which operates at a rate of up to about hundred kHz. It provides opportunity for 3D scanning in TPM with the possibility to correct aberrations independently for every focus position or to achieve refocusing of scattered photons in rapidly decorrelating tissues.
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Walther Akemann, Cathie Ventalon, Jean-François Léger, Benjamin Mathieu, Stéphane Dieudonné, Baptiste Blochet, Sylvain Gigan, Laurent Bourdieu, "Ultra-fast 3D scanning and holographic illumination in non-linear microscopy using acousto-optic deflectors", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510L (18 April 2017); doi: 10.1117/12.2275143; https://doi.org/10.1117/12.2275143
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KEYWORDS
Acousto-optics

3D scanning

Photons

Wavefronts

Modulation

Neurons

Tissues

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