Holographic 3D multi-spot two-photon excitation for fast optical stimulation in brain

Yu Takiguchi; Haruyoshi Toyoda

doi:10.1117/12.2271607

18 April 2017 Holographic 3D multi-spot two-photon excitation for fast optical stimulation in brain

Yu Takiguchi, Haruyoshi Toyoda

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 1025117 (2017) https://doi.org/10.1117/12.2271607
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

We report here a holographic high speed accessing microscope of sensory-driven synaptic activity across all inputs to single living neurons in the context of the intact cerebral cortex. This system is based on holographic multiple beam generation with spatial light modulator, we have demonstrated performance of the holographic excitation efficiency in several in vitro prototype system. 3D weighted iterative Fourier Transform method using the Ewald sphere in consideration of calculation speed has been adopted; multiple locations can be patterned in 3D with single hologram. Standard deviation of intensities of spots are still large due to the aberration of the system and/or hologram calculation, we successfully excited multiple locations of neurons in living mouse brain to monitor the calcium signals.

Citation Download Citation

Yu Takiguchi and Haruyoshi Toyoda "Holographic 3D multi-spot two-photon excitation for fast optical stimulation in brain", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 1025117 (18 April 2017); https://doi.org/10.1117/12.2271607

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