Multi-capillary based optical sensors for highly sensitive protein detection

Yasuhira Okuyama; Takashi Katagiri; Yuji Matsuura

doi:10.1117/12.2275021

18 April 2017 Multi-capillary based optical sensors for highly sensitive protein detection

Yasuhira Okuyama, Takashi Katagiri, Yuji Matsuura

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102511W (2017) https://doi.org/10.1117/12.2275021
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

A fluorescence measuring method based on glass multi-capillary for detecting trace amounts of proteins is proposed. It promises enhancement of sensitivity due to effects of the adsorption area expansion and the longitudinal excitation. The sensitivity behavior of this method was investigated by using biotin-streptavidin binding. According to experimental examinations, it was found that the sensitivity was improved by a factor of 70 from common glass wells. We also confirmed our measuring system could detect 1 pg/mL of streptavidin. These results suggest that multi-capillary has a potential as a high-sensitive biosensor.

Citation Download Citation

Yasuhira Okuyama, Takashi Katagiri, and Yuji Matsuura "Multi-capillary based optical sensors for highly sensitive protein detection", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102511W (18 April 2017); https://doi.org/10.1117/12.2275021

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