A ground based airglow imager interferometer (GBAII) has made in our group to detect the upper atmospheric temperature and wind velocity at terrestrial altitude 90-100 km. GBAII’s sources are the airglow of O (1S) 557.7 nm at altitude of 97km and O2 (0-1) 867.7nm at altitude of 94 km. a uniform value of the atmospheric transmissivity was used in the forward and inversion process of GBAII, which was led to GBAII a lower wind measurement accuracy. The atmospheric transmissivity of O2 (0-1) airglow is calculated and simulated in this paper. Based on the analysis and calculation of the high level atmospheric absorption, scattering attenuation, scattering ratio, acquire transmittance and the attenuation ratio of CO2 and water vapor are obtained by adding the methods of weighted mean, number density of molecules, different height modified segments, the different correction factors vs. different atmospheric heights etc. By MATLAB programme, the atmosphere scattering ratio is obtained to be 1.876×10-3, CO2 transmittance to be 0 and water vapor to be 0.1888, respectively. The total atmospheric transmittance of O2 (0-1) airglow is 0.4663.