An Apparatus For Laser Scanning Microscopy And Dynamic Testing Of Muscle Cells

Ian Hunter; Serge Lafontaine; Poul Nielsen; Peter Hunter

doi:10.1117/12.950333

9 February 1989 An Apparatus For Laser Scanning Microscopy And Dynamic Testing Of Muscle Cells

Ian Hunter, Serge Lafontaine, Poul Nielsen, Peter Hunter

Proceedings Volume 1028, Scanning Imaging; (1989) https://doi.org/10.1117/12.950333
Event: 1988 International Congress on Optical Science and Engineering, 1988, Hamburg, Germany

Abstract

A Type 2 (confocal) transmission laser scanning microscope containing dynamic mechanical testing facilities for the purpose of isolated muscle fiber studies is under development in our laboratory. An embryonic skeletal muscle cell may be kept alive and grown in a temperature-controlled chamber which is confined by two microscope objectives. The muscle cell can be electrically stimulated or manipulated using two motor clusters in 3 dimensions for imaging purposes while independent mechanical experiments are performed. The microscope consists of an interferometer which works either in a Mach-Zehnder configuration to provide cross-correlation functions from which magnitude, phase, and polarization information is obtained, or in a Michelson arrangement to provide auto-correlation functions (eventually for Fourier transform Raman spectroscopy). The microscope is presently controlled by a MicroVAX-II/GPX system while a dedicated parallel computer (multiple-instruction multiple-data) is being developed to cope with the demanding analysis and real-time control require-ments of the apparatus. The approach taken is to avoid acquiring 3-D images and instead to fit structural models to optical data acquired from the original object as needed by the model building algorithms.

Citation Download Citation

Ian Hunter, Serge Lafontaine, Poul Nielsen, and Peter Hunter "An Apparatus For Laser Scanning Microscopy And Dynamic Testing Of Muscle Cells", Proc. SPIE 1028, Scanning Imaging, (9 February 1989); https://doi.org/10.1117/12.950333

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