29 August 2017 Spatial and spectral resolution of photosensitizer fluorescence in cells and tissues
Author Affiliations +
Proceedings Volume 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging; 103132U (2017) https://doi.org/10.1117/12.2283898
Event: Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 2002, Ottawa, Ontario, Canada
Abstract
Spatial and temporal distribution patterns of photosensitizers or their photoproducts is an important observation when attempting to understand the cellular and molecular effects of a particular photochemotherapeutic compound on cells and tissues. While fluorescence microscopy can be readily used to determine the spatial distribution of specific fluorophores within cells, certain limitations arise with this commonly used technique. These limitations include spectral overlap between probes and the fluorophore of interest, endogenous autofluorescence of various intracellular components, and artifactual signals derived from exogenous dyes, all of which interfere with the fluorescent signals emitted from the molecules of interest in a study. The most significant artifactual fluorescent signals given off by intracellular molecules include fluorescence emitted from certain aromatic amino acids, collagen, elastin, pyridoxine, nicotinamide adenine dinucleotides, flavins, and several different porphyrins. In addition to these obstacles, the intercellular environment may influence some fluorophores when macroscopic analysis of tissues is performed. Different fluorophores have different photodynamic activities, photobleaching characteristics, emission wavelength maxima and bandwidths, and fluorescence quantum yields, all of which may lead to a shift in a particular emission signal by a certain fluorophore in a given tissue environment.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Robert P. Watering, Robert P. Watering, } "Spatial and spectral resolution of photosensitizer fluorescence in cells and tissues", Proc. SPIE 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 103132U (29 August 2017); doi: 10.1117/12.2283898; https://doi.org/10.1117/12.2283898
PROCEEDINGS
2 PAGES


SHARE
Back to Top