Two-photon photodynamic therapy: photobleaching rates

Rebecca Goyan

doi:10.1117/12.2283904

29 August 2017 Two-photon photodynamic therapy: photobleaching rates

Rebecca Goyan

Proceedings Volume 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging; 1031330 (2017) https://doi.org/10.1117/12.2283904
Event: Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 2002, Ottawa, Ontario, Canada

Abstract

A promising development in photodynamic therapy (PDT) is the use of two-photon excitation (TPE). The confinement of the excitation volume leads to the possibility of subcellular PDT. Thus, in order to design a treatment protocol, one must be aware of where a photosensitizer localizes in a cell and the individual differences, both between cellular localization sites and individual cells. One way to determine the subcellular location is to observe photobleaching dynamics in cells and compare the rate constants to those observed in solvents which have been chosen to model different characteristics of environments, such as polarity. We have observed the photobleaching behaviour of Verteporfm (VP) in a variety of solvents and single cells and have been able to correlate subcellular position with environmental polarity.

Citation Download Citation

Rebecca Goyan "Two-photon photodynamic therapy: photobleaching rates", Proc. SPIE 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 1031330 (29 August 2017); https://doi.org/10.1117/12.2283904

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