23 April 2017 Double resonance long period fiber grating for detection of E. coli in trace concentration by choosing a proper bacteriophage
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Proceedings Volume 10323, 25th International Conference on Optical Fiber Sensors; 103234X (2017) https://doi.org/10.1117/12.2265950
Event: 25th International Conference on Optical Fiber Sensors, 2017, Jeju, Korea, Republic of
Abstract
There is a critical need of a fast, specific and reliable assay for biological species. To address this need, long period fiber gratings (LPFG) among other fiber optic sensors can be used because of their high sensitivity to changes in surrounding medium. In this work we fabricated and used two over-etched LPFGs. One of them was covered with T4 Phage and the other was covered with MS2 phage that both specifically bind with Escherichia coli (E. coli) bacteria. This bacterium is a major cause of the food contaminations and outbreaks. We showed achieving a highest sensitivity region of the LPFG and the way to fine tune to that region by over-etching the grating. Finally, using the highly sensitive LPFG platform we could detect E. coli at concentrations as low as 100 colony forming units (CFU), by covering the LPFG with an optimized bio-functionalization of the fiber surface with MS2 bacteriophage.
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Y. Chiniforooshan, A. Celebanska, M. Janik, P. Mikulic, F. Haddad, J. Perreault, W. J. Bock, "Double resonance long period fiber grating for detection of E. coli in trace concentration by choosing a proper bacteriophage", Proc. SPIE 10323, 25th International Conference on Optical Fiber Sensors, 103234X (23 April 2017); doi: 10.1117/12.2265950; https://doi.org/10.1117/12.2265950
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